There has been a great demand for skilled and trained gas chromatography experts in various industries in India in the past couple of decades.
As industries gear up to face tightening restrictions on emissions and quality standards, the gas chromatography technique has become the go-to solution for pharmaceutical, healthcare, food & beverage, cosmetics, agricultural production and chemical industries mainly.
In addition, HPLC testing is also mandatory for production of synthetic materials and for performing environmental research. This is a positive sign for aspiring analytical chemists to pursue an HPLC course online from the best technical institutes in India.
What should choose High-Performance Liquid Chromatography (HPLC)?
High-performance liquid chromatography, also known as HPLC in short, is an analytical process that is designed to separate the various components in liquid and gaseous sample materials. It helps to identify the individual components of raw materials and finished goods.
This innovative and advanced testing training is most useful for:
- Quality Assurance (QA) managers
- Practicing chemists
- Research and development (R&D) scientists
- Government quality and environment standard regulatory bodies
- Researchers in life science
- Environmental scientists
The course is designed to ensure ample exposure of students to the advances in the HPLC technology which a skilled and well-trained analytical chemist needs to have today. It covers the basics and advanced studies for practical aspects, especially for beginners in the field.
The online HPLC course of your choice should offer a wider coverage to fundamental HPLC concepts, including laboratory operations in an easy to understand way. This way, experts and beginners will find useful references in the course which is used for performing regular chromatography procedures in industries.
Tips to choose the best HPLC Course Online
As compared to other chromatography techniques, the HPLC technique is held in high regard by researchers, scientists and chemists alike. It is useful for various purposes and is more preferred due to its incredible efficiency, faster speed and excellent accuracy in performing analysis.
Those who hear about HPLC often find it complicated to understand. However, when you choose to attend the best HPLC course online and train with expert teachers you can easily get a solid understanding of its basics and advanced aspects, including the methods and techniques that are involved in performing HPLC tests.
In just a few months, aspirants can learn everything they need to know about the instruments, method of development and validation that are related to HPLC testing. The course will ensure that the student is completely trained and certified to begin working in laboratories that are focused on performing HPLC testing.
If you are looking to join the best online course for HPLC in India, then these are the top 5 things you need to keep in mind before starting the course:
1. HPLC process segregates components in a compound according to their polarity
HPLC is a chromatographic method that separates individual components in a compound sample for identifying, quantifying and purification purposes. This is achieved by first separating the components according to their polarity.
Polar compounds contain positively charged molecules on one end and negatively charged molecules on the other end. On the other hand, non-polar compounds do not have differently charged molecules separated. By nature, polar compounds readily mix with other polar compounds while non-polar compounds remain separated in a mixed compound.
2. HPLC technique uses stationery and mobile phase for separating compounds into individual components
The stationary phase is included in an HPLC column, which you will learn more about when you study the basics in HPLC training. The stationary phase is composed of a non-polar compound. Normally, silica particles are used as a stationary phase. The mobile phase is commonly a mixture of water and any type of organic solvent.
The mobile phase is moved through the HPLC column where the sample compound gets analyzed. The analysis requires using a concentration gradient where the mobile phase being pumped out of the column becomes increasingly non-polar due to being gradually dissolved in the solvent.
3. HPLC tests help to identify individual components in the sample compound
The sample compound will either stick to the non-polar stationary phase in the detector column or are quickly flushed out with the mobile phase as and when their priority matches. The detector measures the compounds being flushed out.
Once the HPLC analysis is completed, the machine generates a chromatogram which details the identities of every individual component in the sample compound.
4. HPLC training has several advantages
Aspirants should be aware of the numerous advantages of choosing an HPLC course online. It is one of the leading analytical techniques in various types of laboratories worldwide. It helps to separate and identify the basic individual components in compounds, from tiny organic molecules to larger, synthetic polymers.
When the HPLC test is combined with mass spectrometry, it enhances separation capabilities and specificity to remarkable levels.
Hopefully, the information provided above has proven the importance and advantage of pursuing an online course for HPLC training India and will help aspiring scientists and chemists to decide the best-suited training institute to get admission into.
About The Author
Ashley Rosa
Ashley Rosa is a freelance writer and blogger. As writing is her passion that why she loves to write blogs related to the latest trends in technology and sometimes on health-tech as well. She is crazy about food.
